Analytical Method Development and Validation for the Simultaneous Estimation of Buprenorphine Hydrochloride and Naloxone Hydrochloride in Pharmaceutical Dosage Forms By RP-HPLC

Authors

  • Katasani Damodar 1Lecturer in Chemistry, Ministry of Education, Eritrea, North East Africa

Abstract

ABSTRACT A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for the simultaneous estimation of Buprenorphine hydrochloride and Naloxone hydrochloride in pharmaceutical dosage forms. Isocratic elution at a flow rate of 1ml min-1 was employed on a symmetry C18 column at ambient temperature. The mobile phase consisted of Water:Methanol:0.1% Ortho phosphoric acid 60:20:20(v/v/v). The UV detection wavelength was at 273 nm. The retention time for Buprenorphine hydrochloride was 3.85 min and Naloxone hydrochloride was 5.85. The method was validated as per the ICH guidelines. The proposed method can be successfully applied for the estimation of Buprenorphine hydrochloride and Naloxone hydrochloride in pharmaceutical dosage forms.

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How to Cite

Damodar, K. (2012). Analytical Method Development and Validation for the Simultaneous Estimation of Buprenorphine Hydrochloride and Naloxone Hydrochloride in Pharmaceutical Dosage Forms By RP-HPLC. International Journal of Pharmaceutical & Biological Archive, 2(6). Retrieved from http://www.ijpba.info/index.php/ijpba/article/view/498

Issue

Vol. 2 No. 6 (2011): International Journal of Pharmaceutical and Biological Archives

Section

Research Articles

License

This is an Open Access article distributed under the terms of the Attribution-Noncommercial 4.0 International License [CC BY-NC 4.0], which requires that reusers give credit to the creator. It allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, for noncommercial purposes only.