A wound is a break in the skin. Wound is usually caused by cuts or scalps, and symptoms at wound or injury include swelling, stiffness, tenderness, discoloration skin tightness, itching, and scar formation, two types of tissue injury. Wound healing is a complex dynamic process. The main objective of this investigation is to study the development, characterization, and isolation of alkaloidal fraction from Tephrosia purpurea and evaluate its wound healing activity in various wound models such as excision, incision, dead space, and burn wound models. Various evaluation parameters such as wound contraction, epithelization time, tensile strength, wet and dry granuloma weight, and hydroxyproline estimation were performed. The main objective of this investigation is to develop a product, which may give a wound healing property, and enhance wound healing process such as increase the collagen synthesis, fibroblast proliferation, angiogenesis, and epithelization because products which are available in market are either antiseptic or antimicrobial.